Diagenode
H3K27ac Antibody
C15410196
Rabbit polyclonal
Info:Supplier Page
Browse Similar Products: H3C7 Antibodies
The acetylation on the Lysine 27 of the histone H3 is a marker of open and active chromatin. This antibody was used to evaluate the chromatin state and establish the epigenetic landscape in different tissues (kidney, adrenal gland).
ChIP
Dissociated, crosslinked tissues ( adrenal gland, kidney)
1% formaldehyde for 10' at RT (crosslink)
Glycin 0,1M final concentration (quenching)
1ug antibody for 5ug sonicated chormatin, ON at 4°C rocking
Protein G coated beads, 30ul/ug of antibody, 2 hours at 4°C rocking
qPCR on precipitated chromatin or sequencing
The antibody reliably defined a precise pattern of histone H3K27 acetylation in different cell types and tissues. The significant enrichment of the precipitated regions showed a narrower distribution compared with the H3K4me3, suggesting a better resolution of the open/active chromatine regulatory regions.
N/A
Specific and strong signal
None
Very good alternative to H3K4me3 antibodies in chromatin immunoprecipitation assays. The quality of this product reflects on the excellent results obtained.