MTT Cell Viability Assay

November 16, 2022

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Company:

Thermo Fisher Scientific (Invitrogen)

Product Name:

MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide)

Catalog Number:

M6494

Is one of the simple assay to evaluate cell viability. It is a rapid colorimetric assay based on the cleavage of the tetrazolium ring of MTT (3-(4,5-dimethylthazolk-2-yl)-2,5-diphenyl tetrazolium bromide) by dehydrogenases in active mitochondria of living cells as an estimate of viable cell number.

Experimental Design and Results Summary

Application

Cell viability

Starting Material

N/A

Tips

NA

Results Summary

The MTT assay is used to measure cellular metabolic activity as an indicator of cell viability, proliferation and cytotoxicity.

DOI or PMID #

N/A

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Summary

The Good

Efficient and specific

The Bad

None, overall the only issues depend on the quality of sample.

The Bottom Line

is commonly used to evaluate cell viability, proliferation and toxicity after a variety of treatment drug or nanoparticle based. Overall is very efficient and sensitive, cheap and friendly to use.

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