Thermo Fisher Scientific
Pierce RIPA buffer
89901
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RIPA Buffer enables efficient sample lysis specifically for cells and protein solubilization while avoiding protein degradation and interference with the proteins′ immunoreactivity and biological activity. RIPA Buffer also results in low background in immunoprecipitation and molecular pull-down assays.
WB, protein assays
N/A
Use very small amount.
RIPA cell lysis reagent is highly effective for protein extraction from a variety of cell types because it contains three non-ionic and ionic detergents. It is very efficient although is not applicable for all the downstream assay.
Efficient and specific
None, overall the only issues depend on the quality of sample.
Overall the product is working well, is efficient also for cell and tissue samples.