Millipore Sigma
anti Trimethyl Histone H3 (Lys4)
07-473
Rabbit polyclonal
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Histone methylations are key markers for the chromatin state. In out study, we used this antibody to establish the epigenetic landscape of a specific cell type, the renal podocytes.
ChIP
Dissociated embryonic kidneys
1ug antibody for 5ug sonicated chormatin, ON at 4°C rocking
None
Protein G coated beads, 30ul/ug of antibody, 2 hours at 4°C rocking
qPCR on ChIP'd chromatin or sequencing
This antibody was first validated by qPCR on know targets (i.e. promoters of SDHA and NPS2). The promising results led us to further investigate the overall H3K4me3 landscape by whole genome sequencing.
N/A
Very specific and ChIP validated.
Excellent antibody for epigenetic studies with canonical ChIP protocol (crosslink and sonication) on the active chromatin marker H3K4me3.