Excellent Antibody Against Alpha Smooth Muscle Actin

LP2M
University of Cote d'Azur (UCA)
Research Scientist

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Company:

Millipore Sigma

Product Name:

Anti-Actin, α-Smooth Muscle antibody, CY3 coupled

Catalog Number:

C6198

Clone Number:

1A4

Smooth actin is expressed by the cells surrounding the arteries and, in the kidney, by the glomerular podocytes. In these structures, it is important to maintain the flexibility of the endothelial structures undergoing pressure variation. This antibody allows evaluating the expression level of this protein on physiological and pathological conditions.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Paraffine embedded renal samples

Primary Incubation

1:500 in blocking solution, 1 hour at RT

Blocking Agent

PBS + donkey normal serum 10%

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence/confocal microscopy

Results Summary

This antibody allowed us to evaluate the expression of this protein by the renal podocytes in pathological conditions, such as high blood pressure, hyperglycemia and chemical damage (bacterial endotoxin).

DOI or PMID #

N/A

Additional Notes

This antibody recognised the alpha subunit.

Image Gallery

Summary

The Good

Directly coupled, can be incubated with secondary antibodies.

The Bad

None

The Bottom Line

This antibody directly coupled with the fluorophore CY3. It gives strong and specific signal of the cytoskeleton component alpha smooth muscle actin.

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