Excellent Marker to Visualize Basal Membrane in Tissues

November 11, 2022

BlueskyReddit
LP2M
University of Cote d'Azur (UCA)
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Millipore Sigma

Product Name:

Anti Laminin

Catalog Number:

L9393

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: Laminin Antibodies

Laminin is a common component of the basal membrane. This antibody is a good choice to visualise the epithelial/endothelial structures within different tissues.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Frozen tissue, paraffine embedded tissue, 3D organs

Primary Incubation

1:200 in blocking solution, 1 hour at RT

Blocking Agent

PBS + donkey normal serum 10%

Secondary Incubation

Fluorescence coupled secondary antibody, 1:500 for 1 hour at RT

Tertiary Incubation

None

Detection

Fluorescence/confocal microscopy

Results Summary

This antibody allowed us to visualise the epithelial/endothelial structures when working both on whole organs (embryonic murin kidneys, thick organs fresh slices - vibratome), fixed-paraffine embedded sections, frozen tissues. It

DOI or PMID #

N/A

Additional Notes

If using PFA fixed-paraffine embedded tissue sections it works better after PK treatment (PK at 5-10 ug/ml in PBS, 15 min RT).

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Summary

The Good

Very flexible and reliable.

The Bad

PK treatment is required for better signal in PFA fixed-paraffine embedded tissues.

The Bottom Line

This antibody is very easy to used and versatile. It can be used in whole mount tissues imaging (fluorescence) , classical immunostaining on paraffine embedded sections (fluorescence or enzymatic detection) and can be also used in tandem after in situ experiments.

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