Reliable Master Mix for Real-Time PCR Workflows

November 11, 2022

BlueskyReddit
Houston Methodist Research Institute
Nanomedicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Applied Biosystems™

Product Name:

PowerUp™ SYBR™ Green Master Mix

Catalog Number:

A25742

We used this PowerUp™ SYBR™ Green Master Mix for real-time PCR for 7 years. It works great to amplifiy targets for accurate gene expression analysis.

Experimental Design and Results Summary

Application

Real-time PCR

Starting Material

N/A

Tips

This is a 2X master mix. Recommended primer concentrations are 300–800 nM.

Results Summary

PowerUp™ SYBR™ Green Master Mix generated single melting curves well, consistent with highly specific amplification.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Reliable

The Bad

Little expensive.

The Bottom Line

Great Master Mix, highly recommended.

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