ChIP-qPCR Kit with Sonication

December 07, 2022

BlueskyReddit
Weill Cornell Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Diagenode Diagnostics

Product Name:

iDeal ChIP-qPCR Kit

Catalog Number:

C01010180

Generally, you can get your ChIP-qPCR results in 3 days: Day 1-2: perform the ChIP step; Day 3: qPCR analysis. A little reminder: with this kit, only up to 4-6 different cell/tissue samples can be made available from start to finish.

Experimental Design and Results Summary

Application

Chromatin immunoprecipitation

Starting Material

Cell lines

Tips

DNA/Chromatin shearing settings need to be optimized beforehand.

Results Summary

For negative control, we got only 0.01% of INPUT Enrichment-- which indicates a good ChIP-- to the best of my knowledge.

DOI or PMID #

N/A

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Summary

The Good

Instruction/protocol is well-written and easy to follow; also this kit is compatible with Bioruptor® Plus sonication device (cat. B01020001; Diagenode Diagnostics).

The Bad

Limited to 4-5 samples from start-to-end, not including one sample used to optimize the DNA/Chromatin shearing settings.

The Bottom Line

Easy-to-use and well-written protocol; pricing is similar to others.

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