User Friendly Fixation Kit

November 10, 2022

BlueskyReddit
UiO
Immunology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Fixation/Permeablization Kit

Catalog Number:

554714

Our lab is using a lot of cell-based assays. As such, we often need to characterize the cells used and their markers, usually by FACs, either as extra or intracellular staining.

Experimental Design and Results Summary

Application

Cell fixation and FACs

Starting Material

Cell lines

Tips

Antibody incubation times can be cut down to ~15min.

Results Summary

This kits allow us to detect intracellular targets using antibody-based approaches or to simply fix cells for later usage.

DOI or PMID #

N/A

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Summary

The Good

Easy to use, not expensive.

The Bad

Long protocol, especially when secondary antibodies are necessary.

The Bottom Line

The kit is easy to use and works well. Background noise can be quite high, so extra washing steps and isotype controls are recommended.

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