Clean Separation of Polypeptides (2-400KDa)

October 31, 2022

BlueskyReddit
MD Anderson Cancer Center - University of Texas
Cancer System Imaging (Diagnostic Imaging Division)
Lab Technician

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Company:

BioRad

Product Name:

4–20% Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 50 µl

Catalog Number:

4561094

I usually used Mini-PROTEAN TGX Precast Protein Gels for the separation of polypeptides in the following from 2KDa up to 400 kDa. I use the standard running buffer containing SDS (10x Tris/Glycine/SDS Running Buffer) or without to retain protein native conformation. I recommend when using Laemmli buffer to dilute 1:1 with sample (and add sterile water to balance all the volumes for a perfect run).

Experimental Design and Results Summary

Application

Separation of polypeptides (Western blot, SDS-page)

Tips

I recommend when using Laemmli buffer to dilute 1:1 with sample (and add sterile water to balance all the volumes for a perfect run).

DOI or PMID #

N/A

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Summary

The Good

Clean separation of polypeptides (2-400 kDa)

The Bad

N/A

The Bottom Line

I usually used Mini-PROTEAN TGX Precast Protein Gels for the separation of polypeptides in the following from 2KDa up to 400 kDa. I use the standard running buffer containing SDS (10x Tris/Glycine/SDS Running Buffer) or without to retain protein native conformation.

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