Excellent Assembly Master Mix for Cloning

October 26, 2022

BlueskyReddit
University of Cote d'Azur (UCA)
LP2M
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

New England Biolabs

Product Name:

NEBuilder HiFi DNA Assembly Master Mix

Catalog Number:

E2621L

We perform a lot of cloning and plasmid modification, and often we are limited by the absence of proper restriction site in the receiving or donor plasmid. This master mix allowed us to bypass this limitation and speed up the cloning procedures.

Experimental Design and Results Summary

Application

Cloning/assembly

Starting Material

Plasmids and PCR products

Tips

The volumes could be scaled down by half without problems.

Results Summary

This kit is very efficient in assembly PCR products into receiving linearised plasmids without the limitation of finding matching restriction sites.

DOI or PMID #

N/A

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Summary

The Good

Easy to use, can be scaled down and save reactives.

The Bad

None

The Bottom Line

Useful master mix that requires only the addition of linearised plasmid and PCR derived insertion/s. The efficiency is very high and almost all the bacteria colonies are positive for the expected insertion.

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