FITC Anti-Human CD3 Flow Cytometry Antibody

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

FITC anti-human CD3 Antibody

Catalog Number:

300305

Clone Number:

HIT3a

Info:Supplier Page View Product Specs

Browse Similar Products: CD3 Antibodies

I wanted to stain the expression of CD3 on the surface of peripheral T cells, so I therefore stained human PBMCs in serial dilutions from 1:25, 1:50, 1:100, 1:200, 1:400 and 1:800 respectively from left to right as seen in the image attached.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC

Primary Incubation

30 minutes at room temperature

Blocking Agent

PBS/BSA

Secondary Incubation

None

Tertiary Incubation

None

Detection

b-530/30

Results Summary

This antibody showed excellent brightness and a very clear positive/negative staining. Recommended titration: 1:100.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very bright.

The Bad

Nothing.

The Bottom Line

Absolutely using going forward.

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