Great Nuclear Marker

CTB
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

Anti-U1 snRNP 70 Antibody (C-3): sc-390899

Catalog Number:

sc-390899

Clone Number:

N/A

Info:Supplier Page View Product Specs

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Was using this anti u1 snRNP70 ab for a experiment where I needed the nuclear fraction of cells. This was an excellent marker easy to optimize with no extraneous bands.

Experimental Design and Results Summary

Applications

Western Blot

Sample

K562, HCT116

Primary Incubation

1:5,000

Blocking Agent

Milk

Secondary Incubation

1:10,000 licor 800 goat anti mouse

Tertiary Incubation

N/A

Detection

LiCor

Results Summary

Helped show that cellular fractionation was getting clean nuclear and cytosol fractions. Great loading control.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to use, clean results.

The Bad

None

The Bottom Line

Great signal, highly recommend.

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