Abcam
PE Anti-C5a-R antibody
ab53434
20/70
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Used extensively studying C5aR1 trafficking in mouse neutrophils. Highly specific, although it does bind to the C5a binding site, so prevents agonist induced internalization once bound.
Flow Cytometry
Mouse Neutrophils
1:60 30 minutes on ice
Fc Block
N/A
YETI flow cytometer
Used extensively studying C5aR1 traffacking in mouse neutrophils. Highly specific, as seen with the dose response curve in Figure 1, where we see dose dependent internalization of C5aR1 in response to increasing C5a concentrations.
Very reliable.
Binds to C5a binding site on receptor.
This antibody has been instrumental in our on-going study of GPRC trafficking in mouse neutrophils.