Great CD4 Antibody for IF

October 20, 2022

BlueskyReddit
Institute of Microbiology, Infectious Diseases and Immunology
Charité – Universitätsmedizin Berlin
Principal Investigator

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor® 488 anti-mouse CD4 Antibody

Catalog Number:

100532

Clone Number:

RM4-5

Info:Supplier Page View Product Specs

Browse Similar Products: CD4 Antibodies

We wanted to detect helper CD4 T cell infiltration in kidney allografts. Therefore we stained for this antibody.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

PLP fixed 20 μm cryosections of mouse kidney

Primary Incubation

1:100 overnight at room temperature

Blocking Agent

1% BSA, 0.3% Triton X, 2% mouse serum, 2% rat serum

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Images were acquired using an inverted Zeiss LSM780 confocal microscope with an 20x 0.8NA air objective.

Results Summary

Great, reproducible staining of kidney-infiltrating CD4 (green) T cells. Blood vessels are stained with CD31 (white).

DOI or PMID #

PMID: 35851547

Additional Notes

N/A

Image Gallery

Summary

The Good

Great CD4 staining

The Bad

None

The Bottom Line

Clone RM4-5 works very well for CD4 staining in mouse cryosections.

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