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Alexa Fluor® 647 anti-mouse CD8a Antibody
100727
53-6.7
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We stained for CD8a, in order to stain for T cell infiltration in kidney allograft and check their localization.
Immunofluorescence
PLP fixed 20 μm cryosections of mouse kidney
1:100 overnight at room temperature
1% BSA, 0.3% Triton X, 2% mouse serum, 2% rat serum
N/A
Images were acquired using an inverted Zeiss LSM780 confocal microscope with an 20x 0.8NA air objective.
Great staining of kidney-infiltrating CD8a+ (red) T cells. Blood vessels are stained with CD31 (white).
PMID: 35851547
Very nice staining.
None
Clone 53-2.1 works great!