Clean Membranes when Blocking with BSA

October 11, 2022

MD Anderson Cancer Center - University of Texas
Cancer System Imaging (Diagnostic Imaging Division)
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Millipore Sigma

Product Name:

Bovine Serum Albumin (BSA)

Catalog Number:

A3059

I usually use BSA for saturating excess protein-binding sites on membranes for Western blotting applications. I tried different concentrations, but the best was 5% BSA in 1X TBS-Tween.

Experimental Design and Results Summary

Application

Western blot

Starting Material

N/A

Tips

5% dilution in 1X TBS-Tween; use it when using phosphor-specific antibodies.

Results Summary

I usually use BSA for saturating excess protein-binding sites on membranes for Western blotting applications. I tried different concentrations, but the best was 5% BSA in 1X TBS-Tween.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Clean results.

The Bad

Expensive

The Bottom Line

When blocking with BSA I always have cleaner membranes compared to non-fat dry milk.

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