Thermo Fisher Scientific
Lipofectamin Stem Transfection Reagent
STEM00001
Info:Supplier Page
We used this reagent transfecting neuronal stem cells. We achieved good transfection without much toxicity.
Transfection
Transfected neuronal stem cells in a 24 wells plate by using 300ng DNA and 2 uL of reagent.
Opti-MEM media is recommended for preparing DNA and transfection reagent complex.
After transfection, we allowed the cells for 24 hours and visualized them under a confocal microscope. Transfection efficiency was ~60%. Cytotoxicity was also minimal.
N/A
Good for transfecting stem cells. Optimization is very easy.
None
Overall it's a good transfection reagent, especially for stem cells. Following manufacturer instruction yields optimum results.