Caspase-3 Assay Kit Works Well

October 07, 2022

Yale University
Internal Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Caspase-3 Assay Kit (Colorimetric)

Catalog Number:

ab39401

Measurement of caspase-3 activity in human and mouse platelets as well as tissue homogenates. Long incubation time and sensitivity of plate reader is important.

Experimental Design and Results Summary

Application

Measurement of caspase-3 activity in platelets and tissue homogenates

Starting Material

Platelet lysates

Tips

Requires long incubation time so plan accordingly. Colorimetric signal does not fade with klonger incubation times.

Results Summary

This helped us determine the intraplatelet caspase-3 activity in both human and mouse platelet lysates following treatment with different drugs and inhibitors.

DOI or PMID #

35862006

Image Gallery

Summary

The Good

Works consistently well. low variability and high reproducibility between different lots.

The Bad

relatively long incubation time and no standards provided.

The Bottom Line

Works well for the price and can be optimized for different samples.

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