RNA Purification from Human Blood Samples Using the Tempus System

October 03, 2022

BlueskyReddit
New York University
Biology
Research Scientist

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Company:

Applied Biosystems (Thermo Fisher Scientific)

Product Name:

Tempus Spin RNA Isolation Kit

Catalog Number:

4378926

We utilize Applied Biosystem's Tempus spin RNA isolation kit to extract and purify RNA from human whole blood samples collected in the Tempus blood RNA tubes for downstream gene expression profiling.

Experimental Design and Results Summary

Application

RNA extraction and purification; Gene expression profiling

Starting Material

Whole blood (human)

Tips

Turn on heat block and refrigerated centrifuge before starting protocol so that they are ready for use once needed later in the workflow.

Results Summary

Workflow ends with a final volume of ~90 ul of purified RNA. The sample should be stored at -80 C (long term) or assayed ASAP.

DOI or PMID #

N/A

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Summary

The Good

Streamlined, easy to follow protocol; Reliable workflow that provides clean output.

The Bad

Workflow requires that samples be collected in specific (Tempus) tubes; Tubes and kit are altogether expensive.

The Bottom Line

We recommend this product for the isolation and purification of total RNA from human whole blood samples for downstream gene expression profiling or other similar applications.

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