SYTOX Blue for Live-Dead Staining

PennState College of Medicine
Pharmacology
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

SYTOX™ Blue Nucleic Acid Stain

Catalog Number:

S11348

We used SYTOX blue for live-dead staining. SYTOX blue stains the permeable dead cells. We used Calcein dye for live cell staining. We analyzed the stained cells under confocal microscopy. We could able to differentiate the live and dead cells.

Experimental Design and Results Summary

Application

Live-dead staining

Starting Material

Mammalian cell culture treated with 1:1000 diluted SYTOX blue.

Tips

None

Results Summary

We observed blue-fluorescently stained dead cells under the confocal microscope. We were able to differentiate them from live cells.

DOI or PMID #

N/A

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Summary

The Good

Easy to use.

The Bad

Staining quality is not very great.

The Bottom Line

Overall it's an average product and can be used for live-dead analysis.

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