Abcam
Anti-CD45 antibody- Hematopoietic Stem Cell Marker
ab30470
[F10-89-4]
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Isolation immune cells from human tissue and checking the cell morphology after seeding in plates for experiments with primary isolated cells.
Immunofluorescence
CD45+ primary cells
1:200, overnight incubation at degree, rocking
3%BSA-PBS
1:1000, 1 hour, mouse AF 594
DAPI in PBS, 5 min
Keyence microscope
This antibody is a good marker to confirm the isolation of CD45+ cells. The dilution, time of incubation worked fine for this type of sample.
N/A
Good quality, easy to use.
None
Antibody with a good signal, good option as a cell marker.