BD Biosciences
BUV395 Rat Anti-Mouse CD8a
563786
53-6.7 (RUO)
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In different healthy and pathological contexts, CD8 T cells differentiate into a range of specialized subsets with different functions. In mouse models of aging and autoimmune diseases we study CD8 T cell diversity. Here, we used anti-CD8a BUV395 to analyze the CD8+ T cell population in healthy control BALB/C mice.
Flow Cytometry
Splenocytes of wild-type 6 months-old BALB/C mouse
Washed twice in PBS and incubated with L/D fixable aqua dye 20 min at 4°C, then washed twice in PBS.
2.4G2
Surface staining with anti-CD3 BUV805, anti-CD4 BUV495, anti-CD8a BUV395 at 1/400, etc., in Cell Staining Buffer (BioLegend) incubated 30 min at 4°C.
Cells are then fixed and permeabilized with commercial kit (True Nuclear, BioLegend) 45 min at 4°C with fixative, and 15 min at 4°C with permeabilization buffer) and stained for transcription factors.
UV laser on Symphony cytometer
The antibody identifies CD8+ T cells with excellent resolution, even after fixation and permeabilization.
N/A
Very bright and robust.
None
Excellent choice to expand a panel with the UV lazer. Expensive, but with the relatively high dilution and quality it compensates!