Ki67 Antibody Used for IF

Pharmacology
UNIVERSITY OF ALABAMA AT BIRMINGHAM
Research Scientist

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Company:

Leica Biosystems

Product Name:

Ki67 antibody

Catalog Number:

#KI67-MM1-L-CE

Clone Number:

N/A

Info:Supplier Page 

Browse Similar Products: Ki-67 Antibodies

Ki67 antibody was used in mouse pancreatic islets by immunofluorescence staining. The result demonstrated that Ki67 was detected in pancreatic duct cell nucleus.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse pancreatic tissue

Primary Incubation

1:500 overnight at 4 degree

Blocking Agent

5% horse serum 1 h at RT

Secondary Incubation

Alexa 488 conjugated secondary antibody

Tertiary Incubation

1:800 1 h at RT

Detection

Olympus Fluorescent Microscope

Results Summary

Ki67 antibody was used to in mouse pancreatic islet by immunofluorescence staining. The result demonstrated that Ki67 was detected in pancreatic duct cell nucleus (green), indicating proliferated cell. DAPI is used for nuclear staining (blues).

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Antibody works well for IF.

The Bad

N/A

The Bottom Line

This antibody works well and highly recommended.

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