Sigma-Aldrich
Anti-HA antibody
H3663
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EK293T cells stably expressing vector or wild-type HA-USP20 were treated with CHX (0.1 mg/ml), and collected at the indicated time points. Cells were lysed and cell lysates were then blotted with the HA antibody. The results showed that USP20 stabilizes β-catenin.
Western Blot
HEK293 whole cell lysates
1/1000 dilution 4°C overnight incubation
5% milk
1/2000 dilution room temperature 1 h incubation
Room temperature 10 min incubation
ECL
https://doi.org/10.1038/s41418-018-0138-z
Fig. 2g
The antibody works well as expected.
The antibody works.