Good Rabbit IgG Antibody

September 22, 2022

BlueskyReddit
pathology
University of Pittsburgh
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

Rabbit IgG Antibody

Catalog Number:

sc-66931

Clone Number:

H-270

Info:Supplier Page View Product Specs

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HEK293T cell were collected and subjected to immunoprecipitation with USP20 or control rabbit IgG and Western blot analysis using β-catenin antibody. The results showed the interaction between endogenous USP20 and β-catenin.

Experimental Design and Results Summary

Applications

Immunoprecipitation

Sample

HEK293 whole cell lysates

Primary Incubation

1/100 dilution 4°C overnight incubation

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

Protein A/G agarose beads

Detection

Western blot

Results Summary

HEK293T cell were collected and subjected to immunoprecipitation with USP20 or control rabbit IgG and Western blot analysis using β-catenin antibody. The results showed the interaction between endogenous USP20 and β-catenin.

DOI or PMID #

https://doi.org/10.1038/s41418-018-0138-z

Additional Notes

Fig. 1b

Image Gallery

Summary

The Good

The antibody works well as expected.

The Bad

N/A

The Bottom Line

The antibody works.

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