Anti-ß-Catenin Antibody

September 22, 2022

BlueskyReddit
pathology
University of Pittsburgh
Research Scientist

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Company:

Abcam

Product Name:

Anti-β-catenin antibody

Catalog Number:

ab16051

Clone Number:

N/A

HEK293T cell were collected and subjected to immunoprecipitation with USP20 or control rabbit IgG and Western blot analysis using β-catenin antibody. The results showed the interaction between endogenous USP20 and β-catenin.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HEK293 whole cell lysates

Primary Incubation

1/1000 dilution 4°C overnight incubation

Blocking Agent

5% milk

Secondary Incubation

1/2000 dilution room temperature 1 h incubation

Tertiary Incubation

Room temperature 10 min incubation

Detection

ECL

Results Summary

HEK293T cell were collected and subjected to immunoprecipitation with USP20 or control rabbit IgG and Western blot analysis using β-catenin antibody. The results showed the interaction between endogenous USP20 and β-catenin.

DOI or PMID #

https://doi.org/10.1038/s41418-018-0138-z

Additional Notes

Fig. 1b

Image Gallery

Summary

The Good

The antibody works well as expected.

The Bad

N/A

The Bottom Line

The antibody works.

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