Great Anti-FLAG Antibody

September 21, 2022

BlueskyReddit
pathology
University of Pittsburgh
Research Scientist

Overall

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Company:

Sigma-Aldrich

Product Name:

Anti-FLAG antibody (M2)

Catalog Number:

F3165

Clone Number:

N/A

Info:Supplier Page 

Browse Similar Products: FLAG Antibodies

HEK293T cells were trabsgected with Flag-β-catenin. Lysates from HEK293T cells expressing Flag-β-catenin were subjected to immunoprecipitation with Flag and Western blot analysis using USP20 antibody. The results showed the interaction between transfected Flag-tagged β-catenin and endogenous USP20.

Experimental Design and Results Summary

Applications

Immunoprecipitation

Sample

HEK293 whole cell lysates

Primary Incubation

1/100 dilution 4°C overnight incubation

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

Protein A/G agarose beads

Detection

Western blot

Results Summary

HEK293T cells were trabsgected with Flag-β-catenin. Lysates from HEK293T cells expressing Flag-β-catenin were subjected to immunoprecipitation with Flag and Western blot analysis using USP20 antibody. The results showed the interaction between transfected Flag-tagged β-catenin and endogenous USP20.

DOI or PMID #

https://doi.org/10.1038/s41418-018-0138-z

Additional Notes

Fig. 1a

Image Gallery

Summary

The Good

The antibody works well as expected.

The Bad

N/A

The Bottom Line

The antibody works.

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