Abcam
Anti-Cytokeratin 18 antibody [C-04]
ab668
N/A
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Currently working with cell culture and doing immunocytochemistry for specific cell markers, such as vimentin, to confirm the cell type after different treatments.
Immunofluorescence
Decidual cells; amnion mesenchymal cells
1:300, overnight incubation at 4 degrees, rocking
3%BSA-PBS
1:1000, 1 hour, mouse AF 594
DAPI in PBS, 5 min
Keyence microscope
The dilution and the incubation time works fine for the cells mentioned above. This antibody is expected to be seen as filaments. Keyence microscope is a good option for this type of antibody.
Easy to optimize.
None
This antibody is a great marker for these types of cells; the dilution works fine.