Good Anti-FLAG Antibody

September 19, 2022

BlueskyReddit
pathology
University of Pittsburgh
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma-Aldrich

Product Name:

Anti-FLAG antibody

Catalog Number:

F1804

Clone Number:

N/A

Info:Supplier Page 

Browse Similar Products: FLAG Antibodies

Caveolin-1–GST fusion protein pull-down assays were performed using cell lysates from NIH 3T3 cells transiently transfected with wild-type Nrf2-flag. Nrf2 directly interacts with caveolin-1 in vitro.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Whole cell lysates

Primary Incubation

1/1000 dilution 4°C overnight incubation

Blocking Agent

5% milk

Secondary Incubation

1/2000 dilution room temperature 1 h incubation

Tertiary Incubation

Room temperature 10 min incubation

Detection

ECL

Results Summary

Caveolin-1–GST fusion protein pull-down assays were performed using cell lysates from NIH 3T3 cells transiently transfected with wild-type Nrf2-flag. Nrf2 directly interacts with caveolin-1 in vitro.

DOI or PMID #

PMC3681691

Additional Notes

DOI: 10.1091/mbc.E12-09-0666

Image Gallery

Summary

The Good

The antibody works well as expected.

The Bad

N/A

The Bottom Line

The antibody works.

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