Y-27632 for Stem Cell Cryopreservation Medium

UNIVERSITY OF ALABAMA AT BIRMINGHAM
Pharmacology
Research Scientist

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Company:

Tocris Bioscience

Product Name:

Y-27632 dihydrochloride

Catalog Number:

No. 1254

I bought Y-27632 dihydrochloride, a selective ROCK inhibitor for stem cell cryopreservation. It's diluted into 10 mM with DMSO first and used with 1:1000 when preparing cryopreservation medium. It froze stem cells very well, and most cells survived when thawing.

Experimental Design and Results Summary

Application

Stem cell cryopreservation

Starting Material

IPS cell ready for freezing

Tips

It's diluted into 10 mM with DMSO first and use 20 ul with 2 ml DMOS and 8ml media when preparing cryopreservation

Results Summary

I bought Y-27632 dihydrochloride, a selective ROCK inhibitor for stem cell cryopreservation. It's diluted into 10 mM with DMSO first and used with 1:1000 when preparing cryopreservation medium. It freezed stem cell very well and most of cell are survived when thawing cell.

DOI or PMID #

N/A

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Summary

The Good

Good for stem cell cryopreservation.

The Bad

N/A

The Bottom Line

it's very good for stem cell survival.

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