Western Blot for ACSL1

September 13, 2022

BlueskyReddit
Pharmacology
UNIVERSITY OF ALABAMA AT BIRMINGHAM
Research Scientist

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Company:

Cell Signaling

Product Name:

ACSL1 antibody

Catalog Number:

#4047

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: ACAS Antibodies

ACSL1 was knockdown in prostate cell line PC3 by shRNA. ACSL1 levels were detected by western blot. Results showed ACSL1 was dramatically decreased in knockdown groups.

Experimental Design and Results Summary

Applications

Western Blot

Sample

human PC3 prostate cell line

Primary Incubation

1:1000 overnight at 4 degree

Blocking Agent

5% milk in TBS

Secondary Incubation

1 hour at room temperature

Tertiary Incubation

1:3000

Detection

ECL

Results Summary

ACSL1 was knockdown in prostate cell line PC3 by shRNA. ACSL1 levels were detected by western blot. Results showed ACSL1 was dramatically decreased in knockdown groups.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Antibody works well.

The Bad

N/A

The Bottom Line

Antibody is pretty specific and highly recommended.

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