Fast Staining for Apoptosis

Robert Bosch Gesellschaft für medizinische Forschung mbH
IKP/RBCT
Graduate Student

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Quality of Results

Ease-of-Optimization

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Company:

Immunotools

Product Name:

Annexin-V-APC-conjugated

Catalog Number:

31490016

We regularly use Annexin-V-APC conjugate for our flow cytometry-based cell death assays and are very satisfied with the results. The staining is quick, not too complicated and if combined with the right flow cytometry setup, very easy to measure and evaluate.

Experimental Design and Results Summary

Application

Cell death detection (apoptosis)

Starting Material

Conventional human (cancer) cell lines - trypsinised and washed

Tips

We stain the cells 10 min on ice before measurement which is already enough time to get good results.

Results Summary

We are testing different apoptosis-inducing drugs and treatments, apoptosis induction after transient transfection etc. e.g. to elucidate the synergism of proteasome inhibition and BCL-2 inhibition via ABT-199 in sarcoma cells

DOI or PMID #

32839432

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Summary

The Good

Quick, easy to handle, decent amount per package.

The Bad

Optimizing the measurement parameters between different cell lines recommended.

The Bottom Line

Perfect if you're in need of an easy and quick cell death assay.

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