NE-PER Nuclear and Cytoplasmic Extraction Reagents Work Well

March 21, 2023

BlueskyReddit
University of Pittsburgh
Surgery
Research Scientist

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Company:

Thermo Fisher Scientific

Product Name:

NE-PER Nuclear and Cytoplasmic Extraction Reagents

Catalog Number:

78833

To induce EMT for BT20 cells, 20 ng/ml EGF and 10 ng/ml TGF-b1 were administered for incubation of 72 h. The cells were collected for fractionation using NE-PER Nuclear and Cytoplasmic Extraction Reagents (78833). The expression levels of Smad3 (cat #9523) was examined to demonstrated for the distribution of the target Smad3. The results showed that Smad3 was primarily located in nuclear fractions.

Experimental Design and Results Summary

Application

20 ng/ml EGF and 10 ng/ml TGFb1 treatment of BT20

Starting Material

BT20

Tips

Sterile technique

Results Summary

To induce EMT for BT20 cells, 20 ng/ml EGF and 10 ng/ml TGF-b1 were administered for incubation of 72 h. The cells were collected for fractionation using NE-PER Nuclear and Cytoplasmic Extraction Reagents (78833). The expression levels of Smad3 (cat #9523) was examined to demonstrated for the distribution of the target Smad3. The results showed that Smad3 was primarily located in nuclear fractions.

DOI or PMID #

N/A

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Summary

The Good

It works.

The Bad

N/A

The Bottom Line

It works well.

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