Great CD3 Antibody

Immunology
UiO
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

BioLegend

Product Name:

Brilliant Violet 421™ anti-human CD3 Antibody

Catalog Number:

300434

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: CD3 Antibodies

Our research focuses on the utilization of T-cell receptors for immunotherapy purposes. We routinely use antibodies against cd3/cd8 for FACs analysis.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

PBMCs

Primary Incubation

1:100, 20 min RT

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSR II

Results Summary

CD3 was used as a selection marker in a killing assay where PBMCs were incubated against a cell line. The separation of the populations was very clear after staining with the antibody.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to use, works wells.

The Bad

A bit expensive.

The Bottom Line

Works well, requires low amounts of antibody and the BV421 fluorochrome gives a strong signal.

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