Cell Signaling
Phospho-STING (Ser366) (E9A9K) Rabbit mAb
50907
E9A9K
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Phosphorylation of STING is a hallmark of cyclic dinucleotide sensing in the cytosol of cells. Here, human myeloid cells were stimulated with poly dA:dT to examine STING activation.
Western Blot
THP-1 cells stimulated over 8 hours with 1ug/ml of poly dA:dT complexed with lipofectamine 2000
1:5000 in TBST+5% milk overnight at 4C
TBST+5% milk for 1 hour
1:10000 goat anti-rabbit (bio-rad) HRP
None
ECL clarity max
This antibody showed an inducible band at the correct molecular weight for STING. Total STING levels were used as an internal control.
N/A
Works well.
Expensive
Expensive but worked well.