Good Alternative to Global Transcriptomics

August 22, 2022

BlueskyReddit
Yale University
Internal Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Qiagen

Product Name:

Human Unfolded Protein Response RT2 Profiler PCR Array System

Catalog Number:

PAHS-089Z

This is a good alternative to global transcriptomics or more expensive RNA sequencing experiments. It analyzes 96 genes related to UPR, allowing a much faster turnaround time. The calculation of results is also made much simpler using the Qiagen online analysis tool.

Experimental Design and Results Summary

Application

Analysis of UPR gene expression in platelets.

Starting Material

Human Platelet samples

Tips

The kit works best while using all reagents from Qiagen.

Results Summary

The kit helped us to analyze the change in expression of UPR related mRNA levels in DM vs HC platelet samples. This helped us determine the presence of these mRNA transcripts as a validation for our protein expression results.

DOI or PMID #

35862006

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Summary

The Good

The kit contains 6 plates and can be used for 6 samples. Eacch qPCR run takes approximate 3.5 hrs and analysis is easy using the Qiagen tool.

The Bad

Have to purchase all reagents from Qiagen and sometime delivery times are slow.

The Bottom Line

The kit worked well for our experiments.

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