Great to Knock-Down Human TRAF3

August 19, 2022

BlueskyReddit
UNC Chapel Hill

Overall

Quality of Results

Ease-of-Optimization

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Company:

Dharmacon

Product Name:

ON-TARGETplus Human TRAF3 siRNA

Catalog Number:

L-005252-00-0005

I have used this SMART-POOL siRNA to Knock-down human TRAF3 protein. It did not need a lot of optimization and honestly worked really well the first time itself! I got around 80-90% KD after 72 hours of transfection.

Experimental Design and Results Summary

Application

Si-RNA mediated KD of human TRAF3

Starting Material

Cultured mammalian cells

Tips

I used lipofectamine as the transfection reagent.

Results Summary

Great to transiently KD human TRAF3. I detected the knockdown using both western blot and qPCR. I used their scrambled control plasmid as control. .

DOI or PMID #

N/A

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Summary

The Good

Easy to use and does the job well.

The Bad

I am very happy with this product. Nothing really bad about it.

The Bottom Line

I really liked this plasmid and highly recommend it. I use a 80% confluent dish of cells and lipofectamine as the transfection reagent.

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