BIP Rabbit Monoclonal Antibody

Yale institute

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

BiP (C50B12) Rabbit mAb

Catalog Number:

#3177

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: BIP Antibodies

The aim of the experiment was to check if the drugs used can cause ER stress. The levels of BiP increase with ER stress. Therefore, in this experiment, the levels of BiP were checked after different drug treatments.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Osteosarcoma cell lines

Primary Incubation

1:1000 in 5% BSA-TBST overnight at 4 degree Celsius

Blocking Agent

5% milk in TBST for 1 h at room temeprature

Secondary Incubation

1:2000 IN 1% MILK-TBST

Tertiary Incubation

N/A

Detection

X RAY FLIMS

Results Summary

the western blot shows that some of the drugs used in this experiment were able to increase the ER stress as could be seen through the enhanced levels of BiP.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very specific antibody, easily detected.

The Bad

The product is not bad.

The Bottom Line

Highly recommended, great antibody specific and easy to detect.

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