Flow Staining Buffers for Transcription Factors

McMaster University
MIRC
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BD Biosciences

Product Name:

Transcription Factor Buffer Set

Catalog Number:

562574

We use this buffer set whenever we want to detect transcription factors in our cells. The buffer set works well to fix and permeabilize the cells to allow access to intracellular antigens, but is gentle enough to maintain intact cells.

Experimental Design and Results Summary

Application

Flow cytometry

Starting Material

Mononuclear cells

Tips

Make sure to use the wash buffer for all washes after permeabilization.

Results Summary

This buffer set works well to fix and permeabilize cells prior to detecting intracellular transcription factors. We are able to detect the transcription factors, while still maintaining surface cell staining.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Easy to use.

The Bad

Expensive

The Bottom Line

Good buffer set for transcription factor staining.

Join the discussion