Liberase for Tissue Digestion

McMaster University
MIRC
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Roche

Product Name:

Liberase TM Research Grade

Catalog Number:

05401119001

I used this reagent for digesting mouse lung tissue to isolate lymphocytes. The reagent is easy to use and provides good results. The lung tissue is adequately digested, while the lymphocytes are still intact with most surface receptors still in place.

Experimental Design and Results Summary

Application

Mononuclear cell isolation

Starting Material

Mouse lung

Tips

Needs to be diluted before use.

Results Summary

This reagent allowed analysis of mouse lung lymphocyte populations, with good yield. Isolated lymphocytes were intact and ready for flow staining.

DOI or PMID #

N/A

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Summary

The Good

Works well every time.

The Bad

Expensive

The Bottom Line

A very good reagent for digesting mouse tissue for lymphocyte isolation.

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