Good CD49a Antibody

MIRC
McMaster University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BV605 Hamster Anti-Rat/Mouse CD49a

Catalog Number:

740375

Clone Number:

Ha31/8

Info:Supplier Page View Product Specs

Browse Similar Products: CD49a Antibodies

I used this antibody to detect and classify mouse NK cells. The antibody gave a decent signal and was able to fit into my panel.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse blood

Primary Incubation

1:100 30 min at 4C

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometer

Results Summary

This antibody was able to stain a relatively rare population of CD49a pbNK cells. The signal was pretty good with minimal bleed into other channels. Due to the unique characteristics of the dye, it was able to work great with my panel.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good staining.

The Bad

Need special reagents if using other BV dyes.

The Bottom Line

Good antibody to fit in a crowded panel.

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