Great Human CD3 Antibody

June 14, 2022

MIRC
McMaster University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

FITC anti-human CD3 Antibody

Catalog Number:

300406

Clone Number:

UCHT1

Info:Supplier Page View Product Specs

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I use this antibody in flow cytometry experiments to detect T cells. The antibody gives a strong signal with little to no background and is quite easy to optimize. It was easy to optimize using the manufacturer's recommended dilution.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

1:20 for 30 min @ 4C

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometer

Results Summary

The antibody gives a strong signal without background staining. It was easy to optimize using the manufacturer's recommended dilution.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good staining.

The Bad

None

The Bottom Line

Highly recommend.

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