BD Pharminogen™
Purified Mouse Anti-p21
556431
N/A
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This antibody was used to detect p21 expression from cell lysates. Western Blot analysis was conducted with 25 ug/mL of protein. Easy to optimize, good sensitivity.
Western Blot
Human endothelial cells
1:1000, overnight@4ºC
5% BSA/TBS
Anti-mouse IgG HRP (#HAF007, R&D systems), 1:5000
Chemiluminescent
This antibody provides a clear band at 21 kDa. The dilution can be reused twice without losing sensitivity.
Easy to optimize, good sensitivity.
It cannot be reused too many times.
The antibody has a great signal.