Santa Cruz Biotechnology
Anti-ubiquitin
sc-8017
P4D1
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A mouse model with I4895T mutation in type1 ryanodine receptor displays muscle weakness and atrophy. Muscle of this mutant model showed decreased SR Ca2+ transient, decreased cytosolic Ca2+, increased mitochondrial ROS, increased ubiquitination, and increased ER stress compared to WT.
Western Blot
Skeletal muscle of I4895T and WT
1:500 for overnight
Bio-Rad EveryBlot Blocking buffer
1:10,000, 1 hour at room temperature (StarBright 700)
No tertiary incubation.
Bio-Rad ChemiDoc MP Imaging System
Ab detects total ubiquitination in the muscle lysates. I4895T of RyR1 mutation greatly increases the total ubiquitination.
10.1038/ncomms14659
N/A
Highly specific.
Not detected.
This Ab was tested by western blot.