Excellent Anti-SIGLEC H Antibody

Houston Methodist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-SIGLEC H antibody

Catalog Number:

ABM2B55

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: SIGLECH Antibodies

The antibody works well in western both at 1:500 and 1:1000 dilution depending on the protein expression in the sample. Very good antibody overall. Recommended for Western blot/ chemiluminescence detection to study protein in cells and tissues. This antibody also allows quantitative analysis of antigen amounts when use with a control or comparing various samples/conditions.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cells and tissue

Primary Incubation

1:500 and 1:1000

Blocking Agent

Milk 5%

Secondary Incubation

1:2000

Tertiary Incubation

N/A

Detection

Chemidoc HRP conjugation Ab

Results Summary

Overall the antibody works well. The antibody resulted very sensitive showing specific signal for Western blot application using from 20 to 40ug of protein per sample. Very satisfied.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very sensitive and specific signal for Western blot application.

The Bad

None, overall the only issues depend on the quality of sample.

The Bottom Line

Antibody showed great signal very well recommended.

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