Excellent Signal From Secondary Antibody

June 09, 2022

BlueskyReddit
Cancer System Imaging (Diagnostic Imaging Division)
MD Anderson Cancer Center - University of Texas
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Bio-Rad

Product Name:

Secondary Antibody (Goat Anti-Rabbit Antibody Conjugated to Horseradish Peroxidase)

Catalog Number:

1662408EDU

Clone Number:

N/A

I used this antibody in WB after diluting 1:5000 in NDFM 5% in TBST for 2 hours. The antibody worked perfectly, giving well-shaped and clean bands.

Experimental Design and Results Summary

Applications

Western Blot

Sample

MDA-MB-231, MDA-MB-436, HELA, SUM-149, SUM-1315, JURKAT, PSN1, MIA PaCa-2

Primary Incubation

5% Not fat milk / TBST 1X, O.N

Blocking Agent

NFDM

Secondary Incubation

Dilution 1:5000 in 5% Not fat milk / TBST 1X

Tertiary Incubation

N/A

Detection

Western Blot

Results Summary

I used this antibody in WB after diluting 1:5000 in NDFM 5% in TBST for 2 hours. Antibody worked perfectly giving well shaped and clean bands.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very clean bands.

The Bad

N/A

The Bottom Line

Secondary Antibody Goat Anti-Rabbit has a great signal and gives you very clean bands.

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