Cell Signaling
mTOR antibody
2972
N/A
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I used mTOR antibody for studying proliferation of neuronal cells SHSY5Y after treatment of amyloid beta by WB. Antibody worked perfectly diluted 1:1000 in BSA 5% overnight and followed by secondary 1:5000 2h. Highly recommended.
Western Blot
SH-SY5Y
1:1000 overnight
5% BSA in TBST 1x
1:5000 2 hours
I used mTOR antibody for studying proliferation of neuronal cells SHSY5Y after treatment of amyloid beta by WB. Antibody worked perfectly diluted 1:1000 in BSA 5% overnight and followed by secondary 1:5000 2h. Suggested blocking agent BSA 5% in TBST 1x Highly recommended.
Very strong and clean bands.
Great signal, recommended.