Great TGFb1 for Fibroblasts Activation

Houston Methodist Academic Institute
Biomimetics
Graduate Student

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Company:

Bio-Techne

Product Name:

Recombinant Human TGF-beta 1 Protein

Catalog Number:

240-B-010/CF

We used this protein to activate fibroblasts and studying fibrosis and desmoplasia. We used a concentration ranging from 25 to 50ng/ml on mrc5 fibroblasts cell lines.

Experimental Design and Results Summary

Application

2D and 3D cell culture treatment.

Starting Material

N/A

Tips

Use at a concentration of 25 to 50 ng/ml and detect biomarkers at d5 and d7.

Results Summary

We successfully activated normal fibroblasts on 2D and 3D culture, monitoring their expression for activated fibroblasts markers FAP and aSMA.

DOI or PMID #

doi.org/10.1002/mabi.202100450

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Summary

The Good

Cells activation achieved.

The Bad

N/A

The Bottom Line

Used for many experiments.

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