Bright F-Actin Staining

June 10, 2022

BlueskyReddit
Biomimetics
Houston Methodist Academic Institute
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

Alexa Fluor™ 555 Phalloidin

Catalog Number:

A34055

Clone Number:

N/A

Info:Supplier Page 

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Staining of ovarian cancer cell lines on 3D sponge scaffolds collagen type I based model to detect cells' shape and penetration in the scaffolds.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

3D collagen type I based scaffold cultured with ovarian cancer cell lines.

Primary Incubation

1:100

Blocking Agent

BSA 1%

Secondary Incubation

None

Tertiary Incubation

None

Detection

IF

Results Summary

We could detect cells F-actin on 3D model clearly to monitor cells shape and migration. clear signal and no background.

DOI or PMID #

https://doi.org/10.3390/cells11050824

Additional Notes

N/A

Image Gallery

Summary

The Good

Reliable and good signal.

The Bad

N/A

The Bottom Line

Good signal and suitable for 3D models staining.

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